Antioxidant activity of Origanum vulgare extract gel. Is it important for oral health?. Preliminary study
DOI:
https://doi.org/10.20453/reh.v31i1.3921Keywords:
antioxidants, free radicals, oral health, Origanum vulgare, oxidative stressAbstract
Objective: To determine the antioxidant activity of the hydroalcoholic extract of Origanum vulgare through DPPH, ABTS and FRAP essays. Material and Methods: The sample collected from Origanum vulgare was dried, reduced in size and placed to macerate 500 grams of dry sample in 1000 mL of 97% ethanol for one week. After time, the macerate was processed through a filter and concentrated in an oven. The formulations were carried out and the dilutions were analyzed with the antioxidant essays. Results: DPPH Method, the 25% gel showed an IC50 of 98.485 mg / mL equivalent to the dilution of 78.789% and for the Trolox® standard, it presented IC50 of 2.48 µg / mL. ABTS Method, the 25% gel formulation presented IC50 of 3.687 mg / mL equivalent to a dilution of 77.75% and for the Trolox® standard it presented IC50 of 2.99 µg / mL, a difference from the other formulations. The results evidenced a relation between the percentage of inhibition and concentration of the samples with an accepted correlation (R2) for the gels at 25%, 50%, 75% of extract of Origanum vulgare and Trolox® of 0.9972; 0.9987 and 0.9986 respectively. FRAP Method, with fast action during the 4 minutes, being 125 mg of extract contained in the gel 25% equivalent to 4mg of Trolox®. Conclusions: It was determined that the antioxidant activity equivalent to Trolox®, with antioxidant assays, with the best average free radical uptake power (Trolox / mg) of extract was present in the gel of Extract of Origanum vulgare 25%.
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Valdés JM, Padrón R, El Ghannam Y. Estrés oxidativo en las enfermedades bucales. Rev Cubana Estomatol. 2016; 53 (4): 1-8.
Bhateja S. Role of antioxidants in oral medicine. Int JPharm Sci Res. 2012; 3 (7):1971-1975.
Singh N, Niyogi R, Mishra D, Sharma M, Singh D. Antioxidants in oral health and diseases: Future Prospects. JDMS. 2013; 10 (3):36-40.
Kesarwala AH, Krishna MC, Mitchell JB. Oxidative stress in oral diseases. Oral Dis. 2016; 22(1):9-18.
Pedraza J, Eugenio D, Molina E. Uso de Activadores Naturales de Nrf2 en diversas condiciones patológicas. En: Cárdenas C, González M, Guevara A, Lara R, Matuz D, Molina E, Torres PV. Mensaje Bioquímico. México DF: Facultad de Medicina, Universidad Nacional Autónoma de México; 2016.p. 211-230.
Guija-Poma E, Inocente-Camones MA, Ponce-PardoJ,Zarzosa-NorabuenaE.Evaluacióndelatécnica 2,2-Difenil-1-Picrilhidrazilo (DPPH) para determinar capacidad antioxidante. Horiz Med. 2015; 15(1): 57-60.
Gómez J, Morales G, Ortiz Y, et al. Disminución del daño nuclear y oxidativo al ADN en pacientes con periodontitis después de la ingesta de ácido fólico. Rev Mex Periodontol. 2015;6(3):121-128.
Rodríguez T, Peña M, Gómez N, Santisteban L, Hernández M. Estrés oxidativo: genética, dieta y desarrollo de enfermedades. Correo Científico Médico de Holguín. 2015; 19(4):690-705.
Oroian M, Escriche I. Antioxidants: Characterization, natural sources, extraction and analysis. Food Res Int. 2015; 74: 10-36.
Hoyos-Arbeláez J, Vázquez M,Contreras-CalderónJ. Electrochemical methods as a tool for determining the antioxidant capacity of food and beverages: A review. Food Chemistry. 2017; 221: 1371-1381.
Fernandes RPP, Trindade MA, Tonin FG, et al. Evaluation of oxidative stability of lamb burger with Origanum vulgare extract. Food Chem. 2017; 233: 101–109.
Portes W, Gardrat C, CastellanA. A comparativestudy ontheantioxidantpropertiesoftetrahydrocurcuminoids and curcuminoids. Tetrahedron 2007; 63 (37): 9092–9099.
Kuskoski EM, Asuero AG, Troncoso AM, Mancini-Filho J, Fett R. Aplicación de diversos métodos químicos para determinar actividad antioxidante en pulpa de frutos. Cienc. Tecnol Aliment. 2005; 25(4): 726-732.
Sonawane SK, Arya SS. Citrullus lanatus protein hydrolysate optimization for antioxidant potential. Food Measure. 2017; 11:1834–1843.
Organización Mundial de la Salud.Salud bucodental. Ginebra: Organización Mundial de la Salud; 2020. (Fecha de acceso: 25 de marzo 2020). Disponible en: https://www.who.int/es/news-room/fact-sheets/detail/oral-health
Granato D, Shahidi F, Wrolstad R. et al. Antioxidant activity, total phenolics and flavonoidscontents: Should weban in vitro screening methods?Food Chem. 2018; 264: 471–475.
Gayoso L, Roxo M, Cavero RY, et al. Bioaccessibility andbiologicalactivityofMelissaofficinalis,Lavandula latifolia and Origanum vulgare extracts: Influence of an in vitro gastrointestinal digestion. Journal of Functional Foods. 2018; 44:146-154.
García-Pérez JS, Cuéllar-Bermúdez SP, de la Cruz-Quiroz R. et al. Supercritical CO2 based tailor made valorization of Origanum vulgare L extracts: A green approachtoextracthigh-valuecompoundswithapplied perspectives. J Environ Manag. 2019; 232:796-802.
Karadag A, Ozcelik B, Saner S. Review of Methodsto Determine Antioxidant Capacity. Food Anal. Methods. 2009;2(1):41–60.
Kedare SB, Singh RP. Genesis and development of DPPHmethodofantioxidantassay.JFoodSciTechnol. 2011;48(4):412-22.
Krishnaiah D, Sarbatly R, Nithyanandam R. A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing. 2011; 89(3): 217-233.
Carbonel KN, Suárez SA, Salas AI. Características fisicoquímicas y capacidad antioxidante in vitro del extracto Gentianella nítida. An Fac Med. 2016; 77(4): 333-7.
Harnly J. Antioxidant methods. J Food Comp Anal. 2017; 64 (Part 2): 145-146.
Granato D, Sousa J, Galvao L, Sávio D. Chemical perspectiveandcriticismonselectedanalyticalmethods usedtoestimatethetotalcontentofphenoliccompounds infoodmatrices.TrendsinAnalyticalChemistry.2016; 80: 266-279.
Cavero S, García-Risco MR, Marín FR, et al. Supercriticalfluidextractionofantioxidantcompounds fromoregano:Chemicalandfunctionalcharacterization viaLC–MS and in vitro assays. J of Supercritical Fluids. 2006; 38(1):62-69.
Moghrovyan A, Sahakyan N, Babayan A, Chichoyan N, Petrosyan M, Trchounian A. Essential Oil and Ethanol Extract of Oregano (Origanum vulgare L.) from ArmenianFlora as a Natural Source of Terpenes, Flavonoids and other Phytochemical swith Antiradical, Antioxidant, Metal Chelating, Tyrosinase Inhibitory and Antibacterial Activity. Curr Pharm Des. 2019; 25(16):1809-1816.
Fierascu RC, Ortan A, Fierascu IC, Fierascu I. In vitro and in vivo evaluation of antioxidant properties of wild-growing plants. A short review. Curr. Opin. Food Sci. 2018; 24:1-8.
Teixeira B, Marques A, Ramos C, et al. Chemical composition and bioactivity of different oregano (Origanum vulgare) extracts and essential oil. J Sci Food Agric. 2013;93(11):2702-14.
Méabed EMH, El-Sayed NM, Abou-Sreea AIB,Roby MHH. Chemical analysis of aqueous extracts of Origanum majorana and Foeniculum vulgare and their efficacy on Blastocystis spp. cysts. Phytomedicine. 2018; 43:158-163.
Chun SS, Vattem DA, Lin YT, Shetty K. Phenolic antioxidants from clonal oregano (Origanum vulgare) with antimicrobial activity against Helicobacter pylori. Process Biochemistry. 2005;40(2):809-816.
Fenoy I. Influencia de los antioxidantes, ascorbato de sodio y alfa tocoferol,en la fuerza de adhesión a esmalte previamente blanqueado con dentífricos blanqueadores. Tesis Doctoral. Barcelona, España: Universitat Internacional de Catalunya; 2017. pp.155.
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