Multiplex measurement of proinflammatory cytokines around miniscrews during orthodontic treatment
DOI:
https://doi.org/10.20453/reh.v32i3.4282Keywords:
Tumor necrosis factor-alpha, interleukin-8, saliva, orthodonticsAbstract
Objective: To determine the levels of proinflammatory cytokines around miniscrews with and without loading, used during orthodontic treatment. Material and Methods: A descriptive longitudinal study was executed with a sample of ten miniscrews inserted in patients that attended a dental clinic. Saliva and peri-implant crevicular fluid samples were taken: at baseline (T0), 24 hours after insertion (T1), 1 week after insertion (T2), 24 hours after loading (T3). The samples obtained were processed by immunoassay and read with a flow cytometer. Results: Data analysis was performed using the SPSS program version 21.0. To verify normal distribution, Kolmogorov-Smirnov test was used, followed by ANOVA to compare and determine statistical significance. Levels of inflammatory mediators in the peri-implant crevicular fluid at T1 had the following order of average values: IL-8>IL-1β >IL-6>TNF-α>IL-
10>IL-12p70. Higher values were found 24 hours post-insertion. Salivary levels found were lower but the previously mentioned order was maintained. Statistically significant intergroup differences were found for IL-1β and IL-8 in the peri-implant crevicular fluid. The Scheffe post hoc test showed that there were no statistically significant differences when making an intragroup pair comparison of each mediator level in a given evaluation time. No statistically significant differences were found in saliva inter and intra-group for all the evaluated inflammatory mediators. Conclusions: The miniscrew loading did not generate an increase of the concentrations of the cytokines greater than the effect caused by the insertion per se. The highest levels of inflammatory mediators were found 24 hours post-insertion of the miniscrew.
Downloads
References
Sari E, Uçar C. Interleukin 1beta levels around microscrew implants during orthodontic tooth movement. Angle Orthod. 2007;77(6):1073-8. doi: 10.2319/100506-405.1
Hamamci N, Acun Kaya F, Uysal E, Yokuş B. Identification of interleukin 2, 6, and 8 levels around miniscrews during orthodontic tooth movement. Eur J Orthod. 2012;34(3):357-61. doi: 10.1093/ejo/cjr019
Meffert RM. Treatment of the ailing, failing implant. J Calif Dent Assoc. 1992;20(6):42-5.
Kao RT, Curtis DA, Richards DW, Preble J. Increased interleukin-1 beta in the crevicular fluid of diseased implants. Int J Oral Maxillofac Implants. 1995;10(6):696-701.
Panagakos FS, Aboyoussef H, Dondero R, Jandinski JJ. Detection and measurement of inflammatory cytokines in implant crevicular fluid: a pilot study. Int J Oral Maxillofac Implants. 1996;11(6):794-9.
Aboyoussef H, Carter C, Jandinski JJ, Panagakos FS. Detection of prostaglandin E2 and matrix metalloproteinases in implant crevicular fluid. Int J Oral Maxillofac Implants. 1998;13(5):689-96.
BD Cytometric Bead Array (CBA) Human Inflammatory Cytokines Kit. Instruction manual. Becton, Dickinson and Company. San Jose, California: BD Biosciences; 2010.
Başaran G, Ozer T, Kaya FA, Hamamci O. Interleukins 2, 6, and 8 levels in human gingival sulcus during orthodontic treatment. Am J Orthod Dentofacial Orthop. 2006;130(1):7.e1-6. doi: 10.1016/j.ajodo.2005.12.027
Serra E, Perinetti G, D’Attilio M, et al. Lactate dehydrogenase activity in gingival crevicular fluid during orthodontic treatment. Am J Orthod Dentofacial Orthop. 2003;124(2):206-11. doi: 10.1016/s0889-5406(03)00407-4
Acun F, Hamamci N, Basaran G, Dogru M, Talo T. TNF-α, IL-β and IL-8 levels in tooth early levelling movement Orthodontic treatment. Journal of International Medical Research. 2010; 3(3):16-21.
Huang D, Bai Z, Shen J, Zhao L, Li J. Identification of tumor necrosis factor receptor-associated factor 6 in the pearl mussel Hyriopsis cumingii and its involvement in innate immunity and pearl sac formation. Fish Shellfish Immunol. 2018;80:335-347. doi: 10.1016/j.fsi.2018.06.035
Tuncer BB, Ozmeriç N, Tuncer C, et al. Levels of interleukin-8 during tooth movement. Angle Orthod. 2005 ;75(4):631-6. Erratum in: Angle Orthod. 2005 May;75(3):497. doi: 10.1043/0003-3219(2005)75[631:LOIDTM]2.0.CO;2
Monga N, Chaurasia S, Kharbanda OP, Duggal R, Rajeswari MR. A study of interleukin 1β levels in peri-miniscrew crevicular fluid (PMCF). Prog Orthod. 2014;15(1):30. doi: 10.1186/s40510-014-0030-4
He W, Zhu H, Liu C. Profiles of inflammation factors and inflammatory pathways around the peri-miniscrew implant. Histol Histopathol. 2021; 9:18336. doi: 10.14670/HH-18-336
Leng SX, McElhaney JE, Walston JD, Xie D, Fedarko NS, Kuchel GA. ELISA and multiplex technologies for cytokine measurement in inflammation and aging research. J Gerontol A Biol Sci Med Sci. 2008;63(8):879-84. doi: 10.1093/gerona/63.8.879.
Downloads
Published
How to Cite
Issue
Section
License
The authors retain the copyright and cede to the journal the right of first publication, with the work registered with the Creative Commons License, which allows third parties to use what is published as long as they mention the authorship of the work, and to the first publication in this journal.
