Remineralizing potential of synthetic saliva: an in vitro study
DOI:
https://doi.org/10.20453/reh.v34i1.5320Keywords:
saliva artificial, erosión dental, remineralización dentalAbstract
Objective: To evaluate the remineralizing effect of synthetic saliva on eroded bovine dental enamel. Materials and methods: 50 enamel blocks were prepared from bovine teeth. The specimens were divided into 5 study groups according to treatment: group A (Colgate Total®, toothpaste containing fluoride), group B (MI Paste Plus®, toothpaste containing casein phosphopeptide, amorphous calcium phosphate [CPP-ACP] with fluoride), group C (Salival® Solution, synthetic saliva), group D (distilled water) and group E (no treatment). All specimens in groups A, B, C and D received 0.5% citric acid attacks for 2 minutes at 0, 8, 24 and 32 hours. After each acid attack, toothpastes corresponding to each treatment group were applied. The degree of mineralization was then evaluated by Raman microscopy and surface microhardness by Vickers microhardness. Results: Regarding the degree of mineralization and surface hardness, no significant differences (p > 0.05) were found in the eroded bovine tooth enamel treated with Colgate Total®, Mi Paste Plus® and Salival® Solution in comparison with the healthy enamel samples. But all presented a significantly higher degree of mineralization and surface hardness than the eroded bovine tooth enamel samples preserved in distilled water (p < 0.05). Conclusion: This in vitro study shows that the synthetic saliva Salival® Solution has a remineralizing potential on eroded bovine enamel.
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Abou Neel EA, Aljabo A, Strange A, Ibrahim S, Coathup M, Young AM, et al. Demineralization-remineralization dynamics in teeth and bone. Int J Nanomedicine [Internet]. 2016; 2016(11): 4743-4763. Disponible en: https://doi.org/10.2147%2FIJN.S107624
Ganss C, Lussi A, Grunau O, Klimek J, Schlueter N. Conventional and anti- erosion fluoride toothpastes: effect on enamel erosion and erosion-abrasion. Caries Res [Internet]. 2011; 45(6): 581-589. Disponible en: https://doi.org/10.1159/000334318
Zafar MS, Ahmed N. The effects of acid etching time on surface mechanical properties of dental hard tissues. Dent Mater J [Internet]. 2015, 34(3): 315-320. Disponible en: https://doi.org/10.4012/dmj.2014-083
Hara AT, Kelly SA, González-Cabezas C, Eckert GJ, Barlow AP, Mason SC, et al. Influence of fluoride availability of dentifrices on eroded enamel remineralization in situ. Caries Res [Internet]. 2009; 43(1): 57-63. Disponible en: https://doi.org/10.1159/000201591
Henostroza G, Henostroza N. Concepto, teorías y factores etiológicos de la caries dental. En: Henostroza G, editor. Diagnóstico de caries dental. Lima: Universidad Peruana Cayetano Heredia; 2005. pp. 13-27.
Espinosa R, Bayardo R, Mercado A, Ceja I, Igarashi C, Alcalá J. Efecto de los sistemas fluorados en la remineralización de las lesiones cariosas incipientes del esmalte, studio in situ. RODYB [Internet]. 2014; 3(1): 14-21. Disponible en: https://www.rodyb.com/wp-content/uploads/2013/12/vol-3-2-REMINERALIZACION1.pdf
Bardow A, Lagerlöf F, Nauntofte B, Tenovuo J. The role of saliva. En: Fejerskov O, Kidds E, editores. Dental caries. The disease and its clinical management. 2.a ed. Oxford: Blackwell; 2008. pp. 190-207.
Al-Obaidi R, Salehi H, Desoutter A, Bonnet L, Etienne P, Terrer E, et al. Chemical & nano-mechanical study of artificial human enamel subsurface lesions. Sci Rep [Internet]. 2018; 8: 4047. Disponible en: https://doi.org/10.1038/s41598-018-22459-7
Kielbassa AM, Shohadai SP, Schulte-Mönting J. Effect of saliva substitutes on mineral content of demineralized and sound dental enamel. Support Care Cancer [Internet]. 2001; 9(1): 40-47. Disponible en: https://doi.org/10.1007/s005200000148
Smith G, Smith AJ, Shaw L, Shaw MJ. Artificial saliva substitutes and mineral dissolution. J Oral Rehabil [Internet]. 2001; 28(8): 728-731. Disponible en: https://doi.org/10.1046/j.1365-2842.2001.00803.x
Hahnel S. Sustitutos de la saliva en el tratamiento de la xerostomía. Quintessenz [Internet]. 2010; 23(10): 531-536. Disponible en: https://www.elsevier.es/es-revista-quintessence-9-articulo-sustitutos-saliva-el-tratamiento-xerostomia-X0214098510886746
Meyer-Lueckel H, Hopfenmuller W, Von Klinggraff D, Kielbassa AM. Microradiographic study on the effects of mucin-based solutions used as saliva substitutes on demineralised bovine enamel in vitro. Arch Oral Biol [Internet]. 2006; 51(7): 541-547. Disponible en: https://doi.org/10.1016/j.archoralbio.2006.01.006
Poggio C, Gulino C, Mirando M, Colombo M, Pietrocola G. Protective effect of zinc-hydroxyapatite toothpastes on enamel erosion: an in vitro study. J Clin Exp Dent [Internet]. 2017; 9(1): e118-e122. Disponible en: https://doi.org/10.4317%2Fjced.53068
Vinod D, Gopalakrishnan A, Subramani SM, Balachandran M, Manoharan V, Joy A. A comparative evaluation of remineralizing potential of three commercially available remineralizing agents: an in vitro study. Int J Clin Pediatr Dent [Internet]. 2020; 13(1): 61-65. Disponible en: https://doi.org/10.5005/jp-journals-10005-1715
Lynch RJM, Smith SR. Remineralization agents: new and effective or just marketing hype? Adv Dent Res [Internet]. 2012; 24(2): 63-67. Disponible en: https://doi.org/10.1177/0022034512454295
Butera A, Maiorani C, Gallo S, Pascadopoli M, Quintini M, Lelli M, et al. Biomimetic action of zinc hydroxyapatite on remineralization of enamel and dentin: a review. Biomimetics [Internet]. 2023; 8(1): 71. Disponible en: https://doi.org/10.3390/biomimetics8010071
Meyer-Lückel H, Kielbassa AM. Influence of calcium phosphates added to mucin- based saliva substitutes on bovine dentin. Quintessence Int [Internet]. 2006; 37(7): 537-544. Disponible en: https://www.quintessence-publishing.com/deu/en/article/839692
Shannon IL, Trodahl JN, Starcke EN. Remineralization of enamel by a saliva substitute designed for use by irradiated patients. Cancer [Internet]. 1978; 41(5): 1746-1750. Disponible en: https://doi.org/10.1002/1097-0142(197805)41:5%3C1746::aid-cncr2820410515%3E3.0.co;2-c
Van der Reijden WA, Buijs MJ, Damen JJ, Veerman EC, Ten Cate JM, Amerongen AVN. Influence of polymers for use in saliva substitutes on de- and remineralization of enamel in vitro. Caries Res [Internet]. 1997; 31(3): 216-223. Disponible en: https://doi.org/10.1159/000262403
Buzalaf MAR, Pessan JP, Honório HM, Ten Cate JM. Mechanisms of action of fluoride for caries control. Monogr Oral Sci [Internet]. 2011; 22: 97-114. Disponible en: https://doi.org/10.1159/000325151
Meyer-Lueckel H, Chatzidakis AJ, Kielbassa AM. Effect of various calcium/phosphates ratios of carboxymethylcellulose-based saliva substitutes on mineral loss of bovine enamel in vitro. J Dent [Internet]. 2007; 35(11): 851-857. Disponible en: https://doi.org/10.1016/j.jdent.2007.08.006
Turssi CP, Lima RQV, Faraoni-Romano JJ, Serra MC. Rehardening of caries-like lesions in root surfaces by saliva substitutes. Gerodontology [Internet]. 2006; 23(4): 226-230. Disponible en: https://doi.org/10.1111/j.1741-2358.2006.00117.x
Vissink A, Gravenmade EJ, Gelhard TB, Panders AK, Franken MH. Rehardening properties of mucin- or CMC-containing saliva substitutes on softened human enamel. Effects of sorbitol, xylitol and increasing viscosity. Caries Res [Internet]. 1985; 19(3): 212-218. Disponible en: https://doi.org/10.1159/000260846
Philip N. State of the art enamel remineralization systems. The next frontier in caries management. Caries Res [Internet]. 2019; 53(3): 284-295. Disponible en: https://doi.org/10.1159/000493031
Bizhang M, Schmidt I, Chun YP, Arnold WH, Zimmer S. Toothbrush abrasivity in a long-term simulation on human dentin depends on brushing mode and bristle arrangement. PLoS One [Internet]. 2017; 12(2): e0172060. Disponible en: https://doi.org/10.1371/journal.pone.0172060
Wiegand A, Begic M, Attin T. In vitro evaluation of abrasion of eroded enamel by different manual, power and sonic toothbrushes. Caries Res [Internet]. 2006; 40(1): 60-65. Disponible en: https://doi.org/10.1159/000088908
Carey CM. Remineralization of early enamel lesions with apatite-forming. Dent J [Internet]. 2023; 11(8): 182. Disponible en: https://doi.org/10.3390/dj11080182
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